Friday, January 18, 2019
Bacteria Growth and Glucose Percentages
Bacterias Growth Affected by discordant Glucose Percentages This lab tested whether or not different glucose levels activated bacterium ingathering. My lab conference and I wanted to figure out, if more glucose was go around on the bacteria surfaces, would there be more bacteria development in the agar surface. My group predicted that with 25% glucose (the highest percentage of glucose), the bacteria result would be the greatest. In order to conduct this experiment, my group had 3 ager plates. unmatchable plate had 0% glucose, the next plate had 5% glucose and the third plate had 25% glucose in it.Once we received all the plates, we light an alcohol burner. We so used a pipet to sterile collect bacteria from the elaboration tubes. We so opened the first ager plate (0% glucose) and transferred 10 drops of bacteria refining onto the plate. Then we did the same for the next 2 plates (5% and 25% glucose). Next, we sterilized the broadcaster by waving it in the flame (alcoho l burner) for 15 seconds. Then, we let the spreader cool for 10 seconds, and then opened the first agar plate and spread the bacteria around the plate by quickly and moderately sliding the spreader back and forth across the plate for 5 seconds.Then we closed the plate and did the same thing for the next 2 plates. Next, we squiffy each plate with a strip of Para film and then placed the plates with bacteria on the side of the room until the following week. after(prenominal) waiting a week, we received our groups ager plates back. We noticed that with our experiment, the about amount of bacteria growth had no glucose in it. Our results were the following with no glucose, there was 25% bacteria growth, with 5% glucose added to the ager plates, there was only 15% bacteria growth, and with 25% glucose added to the ager plates, only 1% of bacteria growth was present.The to the lowest degree amount of bacteria growth contained the largest percentage of glucose. Based off of our groups results, we rout out reject our hypothesis. We realized that our anticipation was not what our results showed us. Our prediction was that 25% glucose would have the most amount of bacteria growth, however we can now conclude that bacteria growth does not need whatever glucose to reproduce. In fact, the more glucose there is, the least amount of bacteria growth occurs. If glucose is present in the bacteria, it is possible for bacteria to reproduce however bacteria will reproduce the greatest when no glucose is present at all.
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